Preparing Tris Buffer | NCBioNetwork.org
Preparing Tris Buffer | NCBioNetwork.org
Nov 9, 2021
ncbionetwork.org
You are preparing a reaction buffer for the alkaline phosphatase assay buffer. Your lab wants to save money and decides to make the assay buffer in-house rather than ordering premade buffer.You find a recipe that reads:Alkaline Phosphatase Assay Buffer50 mL Recipe:1. MgCl2 Final Concentration: 5 mM2. Tween-20 Final Concentration: 0.1%3. NaCl Final Concentration: 100 mM4. Fill with H2O up to 50 mLYou go to your reagent shelf and find the following stock solutions:1. MgCl2 Concentration: 1M
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