Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

It takes time to get the hang of new immunodetection methods, and during this learning period you may well see your share of failed experiments. Christiana Binkley, a PhD student in cellular and ...

Meet the trusted FluorChem TM Western blot imaging systems. Simply load your sample, choose a protocol, and hit expose. Load less protein, use smaller amounts of your precious antibodies, and never ...

I joined the University of North Carolina at Chapel Hill in 2021 as a neuroscience graduate student. That fall, I rotated in Graham Diering’s lab, focusing on the role of sleep on normal development ...

Syngene, a subsidiary of Synoptics Ltd., is a prominent supplier of cutting-edge imaging solutions for life science studies. It focuses on imaging systems for capturing and analyzing images of blots, ...

The western blot may appear to be just another staple lab technique. However, there are some stories surrounding its invention that are definitely worth knowing. This might be news to some: scientists ...

A classic laboratory technique, western blotting, has been used by researchers for decades, and it is nearly obsolete. "Biologists currently are stuck in a time warp—they basically use the same tools ...