In 1980, a landmark paper described restriction fragment length polymorphism (RFLP), a method predateding high volume DNA sequencing, that could be used to identify disease-causing genes. Ray ...

Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...

Adapters consist of a core sequence (black) and an enzyme-specific sequence (red). The enzyme-specific sequence allows the ligation of the adapters to the resulting restriction fragments (green) ...

Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...

The first of these methods was Southern Blotting, developed in 1975 by Edward Southern, and is used to detect the sequence of DNA fragments. Since then, two other methods have been developed, called ...