The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

The basic technology of genetic engineering is artificial cutting, splicing, and combination of genes. A gene is a DNA molecule with a certain function. To accurately cut out the DNA linear molecular ...

Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...

This is a preview. Log in through your library . Abstract Here we report the cloning and preliminary characterization of the Pst I restriction-modification system of Providencia stuartii 164.

The biological function of restriction enzymes is derived from the defense of foreign DNA from invading cells. The restriction-modification system of bacteria makes the DNA bases of bacteria to be ...

In the early 1950s, experiments by two teams of researchers, Salvador Luria working with Mary Human and Joe Bertani working with Jean Weigle, showed that some strains of bacteria were more resistant ...

Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...