In the early 1950s, experiments by two teams of researchers, Salvador Luria working with Mary Human and Joe Bertani working with Jean Weigle, showed that some strains of bacteria were more resistant ...

The biological function of restriction enzymes is derived from the defense of foreign DNA from invading cells. The restriction-modification system of bacteria makes the DNA bases of bacteria to be ...

Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...

Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 48 (November 28, 2017), pp. E10261-E10262 (2 pages) ...

TALENs involve the synthesis of the C-terminal with type II restriction enzyme, Fokl, in order to produce heterodimers that form a double-stranded break (DSB) in DNA. Recent advancements in TALEN ...

Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...

Grapevine dieback, caused by Lasiodiplodia theobromae, is an important trunk disease worldwide. Transformants of L. theobromae were generated in an attempt to identify potential pathogenicity-related ...

"We are able to show that caloric restriction slows down aging by preventing an enzyme, peroxiredoxin, from being inactivated. This enzyme is also extremely important in counteracting damage to our ...

Studies have shown that restricting the intake of calories without reducing the intake of vitamins and minerals slows the signs of aging in a wide range of animals including monkeys, rats and fish, ...