Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...
Restriction enzymes are one of the most important tools in the recombinant DNA technology toolbox. But how were these enzymes discovered? And what makes them so useful? Aa Aa Aa When I come to the ...
New England Biolabs' (NEB) popular web tools, Double Digest Finder and Enzyme Finder, are now available for the iPhone™ and iPod® Touch. These tools to help design restriction enzyme experiments are ...
Most restriction endonucleases use Mg²⁺ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that Bfil, a metal-independent restriction enzyme from the phospholipase D superfamily, ...
The biological function of restriction enzymes is derived from the defense of foreign DNA from invading cells. The restriction-modification system of bacteria makes the DNA bases of bacteria to be ...
Studies have shown that restricting the intake of calories without reducing the intake of vitamins and minerals slows the signs of aging in a wide range of animals including monkeys, rats and fish, ...
I remember clearly the first time I made a supervised trip, as an undergraduate student, to the departmental freezer to obtain a precious aliquot of restriction enzyme. Its real value, however, only ...
"We are able to show that caloric restriction slows down aging by preventing an enzyme, peroxiredoxin, from being inactivated. This enzyme is also extremely important in counteracting damage to our ...
Grapevine dieback, caused by Lasiodiplodia theobromae, is an important trunk disease worldwide. Transformants of L. theobromae were generated in an attempt to identify potential pathogenicity-related ...
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