Current approaches to mapping fork progression in the human genome suffer from drastically low throughput. Here, we introduce ForkML, a nanopore sequencing-based method automatically positioning ...

Researchers at The University of Texas MD Anderson Cancer Center have developed a new imaging method, known as RF-SIRF, that quantitatively detects and maps reversed DNA replication forks with ...

Every time a cell divides, it must copy its DNA with extraordinary precision. But this process is constantly challenged by DNA damage. Among the most dangerous lesions are DNA interstrand crosslinks ...

If you are anything like us, whenever you plan a journey, you spend a remarkable amount of time thinking about the start and ...

DNA repair mechanism: HELQ helicase remodels stalled replication forks into stable structures, preventing harmful mutations under replication stress. Advanced imaging tool: RF-SIRF maps reversed forks ...

The regressed arms of reversed replication forks exhibit structural similarities to one-ended double-stranded breaks and need to be protected against uncontrolled nucleolytic degradation. Here, we ...

Embryonic stem (ES) cells are pluripotent stem cells that can produce all cell types of an organism. ES cells proliferate rapidly and have been thought to experience high levels of intrinsic ...

Mouse ES cells present active origin firing and replication forks that progress slowly throughout S phase. These features are integral aspects of genome replication ensuring genome integrity in ES ...

Researchers at The University of Texas MD Anderson Cancer Center have developed a new imaging method, known as RF-SIRF, that quantitatively detects and maps reversed DNA replication forks with ...

A representative figure showing that HELQ-deficient cells fail to undergo normal fork slowing after MMC (a crosslinking agent) treatment, consistent with defective fork reversal. Every time a cell ...